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宿主来源
Rabbit抗原名称
CD137分子别名
Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB; Ila; Ly63; Tnfrsf9免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P20334克隆号
S-547-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes treated overnight with 3μg/ml Concanavalin A纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu, Ms
CD137, a member of the tumor necrosis factor (TNF) receptor family, is a type 1 transmembrane protein, expressed on surfaces of leukocytes and non-immune cells. CD137 is only expressed on the cell surface after T cell activation. When T cells are activated by Antigen Presenting Cells (APCs), CD137 becomes embedded in CD4+ and CD8+ T cells. CD137 is a costimulatory molecule functioning to stimulate T cell proliferation, dendritic cell maturation, and promotion of B cell antibody secretion. CD137 is also involved in cancer having been found upregulated in cancerous cell lines. CD137/ligand stimulation has been found to lead to stronger anti-tumor responses due to cytotoxic T cell activation and is being examined as a possible anticancer therapy.
流式分析
Flow cytometric analysis of CD137 expression on C57BL/6 mouse splenocytes treated overnight with 3 μg/ml Concanavalin A. C57BL/6 mouse splenocytes treated overnight with 3 μg/ml Concanavalin A (right panel) or untreated (left panel) were stained with Alexa Fluor® 647 Rat Anti-Mouse CD25 Antibody (S0B1545) and SDT FITC Rabbit Anti-Human/Mouse CD137 Antibody at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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