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产品中心 流式试剂&相关 FcR阻断剂

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Rat FcR Blocking Reagent

anti-rat CD32

价格 ￥350.00 （ 1-2周）
货号 S0B80148
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    CD32

  • 分子别名

    anti-rat CD32

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 反应种属 ?

    Rt

  • 阳性样本

    SD rat splenocytes

  • 纯化方式

    Protein G

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    Fc receptor blocking

    FCM

  • 稀释度

    应用 稀释度 推荐种属
    Fc receptor blocking 1μg (2μl/test) per million cells in 100μl volume Rt
背景介绍

  • FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining. Human FcR Blocking Reagent is compatible with flow cytometric staining with anti-human CD16, CD32, and CD64 antibodies.

  • 流式分析

    • Flow cytometric analysis of SD rat splenocytes labeled with Rat CD32 antibody at 1/50 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 – PE antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.

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