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宿主来源
Rat抗原名称
TOX分子别名
Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; KIAA0808细胞定位
NucleusAccession
O94900克隆号
S-4516抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse thymocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Ms
TOX protein is a transcription factor that plays a crucial role in the immune system. It is involved in the regulation of T cell differentiation and function, particularly in the development and maintenance of regulatory T cells (Tregs) which are essential for maintaining immune tolerance and preventing autoimmunity. Additionally, TOX has been implicated in the response of T cells to chronic antigen stimulation, such as in the context of chronic infections or cancer, where it may contribute to T cell exhaustion. Its expression and activity can influence the balance between effector and regulatory T cell populations, thereby having a significant impact on the overall immune response.
流式分析
Flow cytometric analysis of TOX expression in C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Pacific Blue™ Rat Anti- Mouse CD8 Antibody and either Biotin Rat IgG1, k Isotype Control (left panel) or SDT Biotin Rat Anti- Human/Mouse TOX Antibody (right panel) at 5 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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