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宿主来源
Rat抗原名称
IL-10分子别名
Interleukin-10; IL-10; Cytokine synthesis inhibitory factor (CSIF); IL10细胞定位
SecretedAccession
P22301克隆号
S-3081抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Th2 polarized human PBMC纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:200 Hu
Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating immune responses. It is primarily produced by various immune cells, including monocytes, macrophages, and regulatory T cells. IL-10 has potent anti-inflammatory properties, as it inhibits the production of pro-inflammatory cytokines such as IL-1, TNF-α, and IFN-γ from activated macrophages and T helper 1 cells. By doing so, it helps to prevent excessive inflammation and tissue damage. Additionally, IL-10 promotes the differentiation of regulatory T cells and enhances the function of B cells, contributing to the maintenance of immune homeostasis. Dysregulation of IL-10 production has been implicated in various diseases, including autoimmune disorders and certain types of cancer, highlighting its importance in both health and disease.
流式分析
Flow cytometric analysis of IL-10 expression in Human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (right panel) or unstimulated (left anel).The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then labeled Human IL-10 antibody at 1/200 dilution (1 μg). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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