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宿主来源
Mouse抗原名称
CD52分子别名
CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5 (He5); HE5细胞定位
Cell membraneAccession
P31358克隆号
S-R445抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Human Peripheral Blood cells纯化方式
Protein A浓度
0.5 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 2μl per million cells in 100μl volume Hu
CD52 is a small glycoprotein consisting of 12 amino acids, anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. It is widely expressed on the surface of various immune cells, including normal and malignant T and B lymphocytes, monocytes, and some epithelial cells. Although its exact biological function remains unclear, CD52 is believed to play roles in T-cell migration, activation, and immune regulation. Importantly, CD52 is the target of alemtuzumab, a humanized monoclonal antibody used to treat diseases like chronic lymphocytic leukemia (CLL) and multiple sclerosis. Alemtuzumab induces cell destruction through mechanisms such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Recent studies also suggest that CD52 may have immunosuppressive effects by binding to the SIGLEC-10 receptor and inhibiting T-cell function.
流式分析
Flow cytometric analysis of Human CD52 expression on human peripheral blood cells. Human peripheral blood cells were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody and either Pacific Blue Mouse IgG2a, κ Isotype Control (left panel) or SDT Pacific Blue Mouse Anti-Human CD52 Antibody (right panel) at 2μl/test. Total viable cells, as determined by Fixable Viability Dye 583 (S0B88803), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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