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宿主来源
Mouse抗原名称
IL-22分子别名
Interleukin-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor (IL-TIF); ILTIF; ZCYTO18; IL22细胞定位
SecretedAccession
Q9GZX6克隆号
S-3255抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Th1 Polarization Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
Interleukin-22 (IL-22) is a 20 kDa α-helical cytokine of the IL-10 family that is produced chiefly by activated innate lymphoid cells, Th17, Th22 and γδ T cells, signals exclusively through a heterodimeric receptor complex of IL-22R1 and IL-10R2 to activate STAT3 and, to a lesser extent, STAT1 and STAT5, and functions as a frontline defender of barrier organs by up-regulating antimicrobial peptides (e.g., β-defensins, RegIIIγ), mucins and tight-junction proteins, stimulating epithelial proliferation and survival, while simultaneously suppressing inflammatory cytokine release from tissue-resident immune cells, thereby orchestrating tissue repair, containment of commensal microbes and protection against extracellular bacterial and fungal pathogens; however, its dysregulation contributes to psoriasis, inflammatory bowel disease, fibrosis and certain cancers, making it a key target for therapeutic modulation.
流式分析
Flow cytometric analysis of IL-22 expression in human PBMC polarized with Th1 Polarization Kit, Human (UA090016). Th1 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Phycoerythrin Mouse Anti-Human CD4 and SDT FITC Mouse Anti-Human IL-22 Antibody at 1.25μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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