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宿主来源
Mouse抗原名称
CD73分子别名
5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase; NT5; NTE; NT5E免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P21589克隆号
S-715-115抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD73, also known as ecto-5'-nucleotidase (encoded by NT5E), is a glycosylphosphatidylinositol (GPI)-anchored cell-surface enzyme that catalyzes the final step of extracellular ATP degradation, converting AMP to immunosuppressive adenosine, thereby orchestrating tumor immune evasion, promoting angiogenesis and metastasis, and shaping an anti-inflammatory microenvironment by dampening CD8⁺ T-cell cytotoxicity and enhancing Treg or myeloid suppressive functions across multiple solid cancers.
流式分析
Flow cytometric analysis of human CD73 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody and either PE/Cy7 Mouse IgG2b, κ Isotype Control (left panel) or SDT PE/Cy7 Mouse Anti-Human CD73 Antibody (right panel) at 1.25μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Beckman CytoFlex S and FlowJo™ software.
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