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Rabbit抗原名称
CD8α分子别名
T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; Lyt-2; Lyt2; Cd8a免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P01731克隆号
S-142-52抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD8α is a crucial immune glycoprotein that serves as a co-receptor for the MHC I-peptide complex, facilitating the recognition of antigens presented by APCs (antigen-presenting cells) to T cells. It is a key component of the CD8 receptor complex, which is predominantly found on cytotoxic T lymphocytes (CTLs). CD8α can form either an α/β heterodimer with CD8β or an α/α homodimer, both of which play essential roles in CTL activation and function. By interacting with the TCR (T cell receptor) and MHC I molecules, CD8α helps recruit the LCK kinase, initiating multiple signaling pathways that drive the CTL-mediated immune response.
流式分析
Flow cytometric analysis of mouse CD8α expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 Antibody and either FITC Rabbit IgG Isotype Control (left panel) or SDT FITC Rabbit Anti-Mouse CD8α Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 583 (S0D0015), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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