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宿主来源
Rat抗原名称
CD206 (MMR)分子别名
Macrophage mannose receptor 1; Mrc1细胞定位
Cell membraneAccession
Q61830克隆号
S-R498抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
BALB/c mouse peritoneal exudates cells纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 0.25μg per million cells in 100μl volume Ms
CD206, also known as the mannose receptor (MR) or mannose receptor C type 1 (MRC1), is a transmembrane glycoprotein that belongs to the C-type lectin family. It is predominantly expressed by tissue macrophages, dendritic cells, and certain lymphatic or liver endothelial cells. CD206 recognizes and mediates endocytosis of pathogens by binding to glycoproteins terminated with mannose. This receptor serves as a homeostatic receptor by binding and scavenging unwanted high mannose N-linked glycoproteins as well as pituitary hormones from the circulation. In addition, CD206 is a marker for the M2 phenotype of macrophages, which are involved in immunosuppression, tissue remodeling, and Th2 effector responses. Its expression is regulated by various cytokines and is considered a marker of an M2-like macrophage phenotype.
流式分析
Flow cytometric analysis of mouse CD206 (MMR) expression on 4% paraformaldehyde fixed and 0.1% Tween permeabilized BALB/c mouse peritoneal exudates cells. The cells were then stained with Pacific Blue™ Rat Anti-Mouse F4/80 Antibody and either APC Rat IgG2a, κ Isotype Control (left panel) or SDT APC Rat Anti-Mouse CD206 (MMR) Antibody (right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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