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Mouse抗原名称
CD54分子别名
Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1细胞定位
MembraneAccession
P05362克隆号
S-3807抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.1 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μl per million cells in 100μl volume Hu
CD54 is an 85–110 kDa transmembrane glycoprotein of the immunoglobulin superfamily that is expressed on endothelial, epithelial and immune cells; upon binding its ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) it serves as the critical adhesion checkpoint that promotes firm leukocyte arrest on the vessel wall, enables subsequent diapedesis into inflamed tissue, and amplifies antigen-specific T-cell activation, thereby positioning it at the mechanistic center of inflammatory, autoimmune and anti-tumor immune responses.
流式分析
Flow cytometric analysis of human CD54 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD19 Antibody and either PE Mouse IgG2a Isotype Control (left panel) or SDT PE Mouse Anti-Human CD54 Antibody (right panel) at 1 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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