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宿主来源
Rat抗原名称
CD45R/B220分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc细胞定位
Cell membrane, SynapseAccession
P06800克隆号
RA3.3A1/6.1抗体类型
Rat mAb抗体同种型
IgM,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Ms
CD45R/B220 is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45R/B220 is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with anti-mouse CD45R/B220 antibody at 1/200 dilution (1 μg) (right panel) compared to a Rat IgM, κ Isotype Control (left panel). Goat Anti-Rat IgM Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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