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宿主来源
Mouse抗原名称
T-Bet分子别名
T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM; TBET; TBLYM; TBX21细胞定位
NucleusAccession
Q9UL17, Q9JKD8克隆号
S-4477抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
T-bet (T-box transcription factor TBX21) is a master regulator protein essential for the differentiation and function of T helper 1 (Th1) cells, playing a pivotal role in the adaptive immune response against intracellular pathogens such as viruses and bacteria. Encoded by the TBX21 gene, this nuclear transcription factor binds to specific DNA sequences to activate the expression of hallmark Th1 cytokines, most notably interferon-gamma (IFN-γ), while simultaneously repressing the development of alternative T cell lineages like Th2 and Th17 by inhibiting their respective lineage-defining transcription factors (GATA3 and RORγt). Beyond its critical function in CD4+ T cells, T-bet also governs the maturation and cytotoxic potential of CD8+ T cells and natural killer (NK) cells, thereby orchestrating a robust cell-mediated immunity; however, its dysregulation or overexpression is implicated in various autoimmune disorders and chronic inflammatory conditions, making it a significant target for immunotherapeutic interventions aimed at modulating immune balance.
流式分析
Flow cytometric analysis of T-Bet expression in PBMC (Human peripheral blood mononuclear cells). Human PBMC were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human T-Bet Antibody at 5 μl/test followed by Sav-iFluor 488. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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