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宿主来源
Rat抗原名称
c-Kit (CD117)分子别名
Mast/stem cell growth factor receptor Kit; SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; Sl; Kit细胞定位
Cytoplasm, Cell membraneAccession
P05532克隆号
ACK2抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.5 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
c-Kit (CD117) is a receptor tyrosine kinase encoded by the c-kit gene. It plays a crucial role in the development and function of various cell types including hematopoietic cells, melanocytes, and gastrointestinal interstitial cells. The ligand for c-Kit is stem cell factor (SCF), and the binding of SCF to c-Kit activates intracellular signaling pathways that promote cell proliferation, survival, differentiation, and migration. Mutations or overexpression of c-Kit are associated with several diseases such as gastrointestinal stromal tumors (GISTs) and certain types of leukemia, making it an important target for therapeutic interventions.
流式分析
Flow cytometric analysis of mouse CD117 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Brilliant Violet 421™ Rat Anti-Mouse Lineage Cocktail Antibody and either Biotin Rat IgG2b, κ Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse c-Kit (CD117) Antibody (right panel) at 2μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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