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宿主来源
Mouse抗原名称
CD328分子别名
Sialic acid-binding Ig-like lectin 7; Siglec-7; Adhesion inhibitory receptor molecule 1 (AIRM-1); CDw328; D-siglec; QA79 membrane protein; p75; AIRM1; SIGLEC7细胞定位
MembraneAccession
Q9Y286克隆号
S-3029抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD328, also known as Siglec-7, is a 467-amino-acid inhibitory receptor of the CD33-related Siglec family that displays a single N-terminal V-set immunoglobulin domain for binding α2,3- and α2,6-linked sialic acids, followed by two C2-set Ig domains, a transmembrane segment and a cytoplasmic tail containing two ITIMs; expressed predominantly on NK cells, CD8⁺ T cells and monocytes, it recruits SHP-1/2 phosphatases upon engagement of sialylated glycans, thereby dampening cytotoxicity, cytokine release and cellular activation to help maintain immune tolerance.
流式分析
Flow cytometric analysis of human CD328 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with APC Mouse Anti-Human CD56 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD328 Antibody (right panel) at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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