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宿主来源
Rat抗原名称
Ig, κ Light Chain分子别名
Immunoglobulin kappa constant; Igkc细胞定位
Cell membraneAccession
P01837克隆号
S-4252抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Ms纯化方式
Protein G浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
Immunoglobulin kappa (Igκ) light chains are one of the two types of polypeptide chains, alongside lambda chains, that constitute the antigen-binding fragment of antibodies in humans and many other mammals, playing a critical role in the adaptive immune system's ability to recognize diverse pathogens. Each antibody molecule typically comprises two identical heavy chains and two identical light chains, where the kappa light chain consists of a variable domain (Vκ) responsible for antigen specificity through hypervariable complementarity-determining regions (CDRs), and a constant domain (Cκ) that provides structural stability and facilitates proper folding and assembly with the heavy chain within the endoplasmic reticulum of B cells. In humans, the immunoglobulin light chain locus is located on chromosome 2, and during B cell development, V-J recombination generates immense diversity in the Vκ region, allowing the immune system to produce billions of unique antibodies; notably, each B cell expresses exclusively either kappa or lambda light chains, a phenomenon known as allelic exclusion, which ensures monospecificity, with kappa chains being more prevalent than lambda chains in human serum at an approximate ratio of 60:40. Clinically, the detection of free kappa light chains or an abnormal kappa-to-lambda ratio is a vital diagnostic marker for B-cell malignancies such as multiple myeloma and light chain amyloidosis, as clonal proliferation often results in the overproduction of a single type of light chain that can be measured in blood or urine to monitor disease progression and treatment response.
流式分析
Flow cytometric analysis of mouse Ig, κ Light Chain Antibody expression on Human PBMC. Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody(S0B8251) and either FITC Rat IgG1, κ Isotype Control (left panel) or FITC Rat Anti-Mouse Ig, κ Light Chain Antibody (right panel) at 0.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.







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