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宿主来源
Mouse抗原名称
IFN-γ分子别名
Interferon gamma; IFN-gamma; Immune interferon; IFNG细胞定位
SecretedAccession
P01579克隆号
S-3076抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Th1 Polarization Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1.25μl per million cells in 100μl volume Hu
Interferon gamma (IFN-γ) is a dimerized soluble cytokine and the only member of the type II class of interferons. It is primarily produced by CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The IFN-γ protein is encoded by the IFNG gene in humans and consists of approximately 160 amino acids, forming a noncovalently linked homodimer with a molecular weight of about 50 kDa. It plays a crucial role in regulating both innate and adaptive immunity by activating signaling pathways, such as the JAK-STAT pathway, in cells like macrophages and B cells. IFN-γ has antiviral, antibacterial, and antitumor properties and is involved in promoting inflammation and cell proliferation. However, its pleiotropic nature also means it can induce immunosuppressive effects, which limit its clinical use.
流式分析
Flow cytometric analysis of IFN-γ expression in human PBMC polarized with Th1 Polarization Kit, Human (UA090016). Th1 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A.
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with PE Mouse Anti-Human CD4 and either PE-Cy7 Mouse IgG1, κ Isotype Control (left panel) or SDT PE/Cy7 Mouse Anti-Human IFN-γ Antibody (right panel) at 1.25μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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