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产品中心 流式试剂&相关 流式抗体

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Mouse IgG3, κ Isotype Control (S-4438)

价格 ￥350.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B80015
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 克隆号

    S-4438

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG3,k

  • 纯化方式

    Protein A

  • 浓度

    0.2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    FCM

背景介绍

  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) labeled with Mouse IgG3, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

    • Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Mouse IgG3, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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