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Rat抗原名称
CD24分子别名
Signal transducer CD24; Lymphocyte antigen 52 (Ly-52); M1/69-J11D heat stable antigen (HSA); Nectadrin; R13-Ag; X62 heat stable antigen; Cd24a; Ly-52; Cd24细胞定位
Cell membraneAccession
P24807克隆号
M1/69抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Ms阳性样本
BALB/c mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 0.25μg per million cells in 100μl volume Ms
CD24, also known as the cluster of differentiation 24, is a highly glycosylated glycosylphosphatidylinositol (GPI)-anchored surface protein that plays crucial roles in various physiological and pathological processes. Initially identified as a B-cell differentiation antigen, it is widely expressed in hematopoietic stem cells, B and T lymphocytes, epithelial cells, and neural cells. Structurally, CD24 has an intracellular domain, a transmembrane domain, and a heavily glycosylated extracellular domain, which is involved in cell adhesion, migration, differentiation, and apoptosis.
流式分析
Flow cytometric analysis of CD24 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Pacific Blue™ Rat Anti-Mouse CD3 Antibody(S0B1539) and either PerCP-Cy5.5 Rat IgG2b, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD24 Antibody (right panel) at 1.25μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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