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Mouse抗原名称
Osteopontin分子别名
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1 (SPP-1); Urinary stone protein; Uropontin; BNSP; OPN细胞定位
SecretedAccession
P10451, P10923克隆号
S-5550抗体类型
Mouse mAb抗体同种型
Mouse IgG2c, κ反应种属 ?
Hu, Ms纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo neutralization
in vitro neutralization
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
Osteopontin (OPN) is a multifunctional, highly acidic, calcium-binding phosphoprotein expressed ubiquitously in tissues and body fluids, where it acts as a matricellular cytokine and signaling molecule that orchestrates diverse biological processes including bone remodeling through its role as a key regulator of osteoclast function, immune responses by promoting Th1 differentiation and macrophage activation, wound healing via cell adhesion and migration, and pathological conditions such as tumor metastasis, chronic inflammation, fibrosis, and cardiovascular calcification by interacting with integrin receptors (αvβ1, αvβ3, αvβ5, αvβ6, α8β1) and CD44 variants through its conserved integrin-binding RGD motif and cryptic domains, thereby activating downstream signaling cascades like PI3K/Akt, MAPK/ERK, and NF-κB pathways that modulate gene expression for cell survival, proliferation, and tissue repair.
免疫印迹
WB result of Invivo anti-Mouse/Human osteopontin (SPP1) Recombinant mAb
Primary antibody: Invivo anti-Mouse/Human osteopontin (SPP1) Recombinant mAb at 1/1000 dilution
Lane 1: OPN/SPP1 His Tag Protein, Mouse lysate 1 µg
Lane 2: OPN/SPP1 His Tag Protein, Human lysate 1 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45-55 kDa
Observed MW: 65, 55 kDa
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