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宿主来源
Human抗原名称
CD274分子别名
Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; B7 homolog 1 (B7-H1); B7H1; PDCD1L1; PDCD1LG1; PDL1; CD274细胞定位
Cell membraneAccession
Q9NZQ7抗体类型
Recombinant mAb抗体同种型
IgG1反应种属 ?
Hu纯化方式
Protein A浓度
5 mg/ml内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
Blocking of PD-L1 signaling Functional assays
FCM
ELISA
稀释度
应用 稀释度 推荐种属 FCM 1:500 Hu
CD274, also known as PD-L1 (Programmed Death-Ligand 1), is a ~33 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily that functions as the key ligand for the inhibitory receptor PD-1 on T cells; upon IFN-γ–driven up-regulation on dendritic cells, macrophages, normal epithelia and many tumors, its engagement of PD-1 delivers a “stop” signal that blocks T-cell receptor signaling, suppressing proliferation, cytokine production and cytotoxic activity, thereby maintaining peripheral tolerance and preventing autoimmunity, but is exploited by cancers to evade immune destruction, making the PD-1/PD-L1 axis a central target for modern checkpoint-inhibitor immunotherapies.
流式分析
Flow cytometric analysis of 293T (Human embryonic kidney epithelial cell, left) / MDA-MB-231 (Human breast adenocarcinoma epithelial cell, right) labeled with anti – Human PD-L1 antibody at 1/500 dilution (1 μg) / (red) compared with a Human IgG1 (black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Human IgG Alexa Fluor® A488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Negative control: 293T
ELISA
Immobilized PD-L1 His Tag Protein, Human (UA010001) can bind Socazolimab. The EC50 for this effect is 3.989 ng/mL.
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