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宿主来源
Armenian hamster抗原名称
TNF alpha分子别名
Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a); Tnfa; Tnfsf2; Tnf细胞定位
Secreted, Cell membraneAccession
P06804, P16599克隆号
S-5535抗体类型
Recombinant mAb同型对照
S0B7062反应种属 ?
Ms, Rt, Rb纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo blocking
in vitro blocking
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt, Rb
Tumor necrosis factor alpha (TNF alpha) is a multifunctional cytokine that plays a crucial role in regulating immune responses and inflammation. It is primarily produced by macrophages, but can also be synthesized by other cell types including monocytes, neutrophils, and T cells. TNF alpha exerts its effects by binding to two distinct receptors, TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2), which are widely expressed on various cell types. It is involved in a wide range of biological processes such as promoting inflammation, inducing apoptosis, and stimulating cell proliferation and differentiation. In the context of immune response, TNF alpha helps to activate immune cells and enhance their ability to combat infections and cancer cells. However, excessive or uncontrolled production of TNF alpha can lead to chronic inflammatory diseases like rheumatoid arthritis, inflammatory bowel disease, and psoriasis. Therefore, TNF alpha is a key target for therapeutic interventions aimed at modulating the immune system and treating inflammatory disorders.
免疫印迹
WB result of Invivo anti-Mouse/Rat/Rabbit TNFα Recombinant mAb
Primary antibody: anti-Mouse/Rat/Rabbit TNFα Recombinant mAb at 1/1000 dilution
Lane 1: TNF-α Protein, Human lysate 1 µg
Lane 2: TNF-α Protein, Mouse lysate 1 µg
Lane 3: TNF-α Protein, Rat lysate 1 µg
Secondary antibody: Goat Anti-Armenian hamster IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
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