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Rat抗原名称
IFNγ分子别名
Interferon gamma; IFN-gamma; Ifng细胞定位
SecretedAccession
P01580克隆号
S-5416抗体类型
Rat mAb同型对照
S0B0760反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro neutralization
in vivo neutralization
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
Interferon-gamma (IFNγ) is the signature cytokine of type II interferon, a 20–25 kDa glycoprotein homodimer that is synthesized and secreted primarily by activated T cells, NK cells and NKT cells; upon binding to its high-affinity receptor (IFNGR1/2) it triggers JAK1/JAK2-mediated phosphorylation of STAT1, launching a transcriptional program that up-regulates MHC class I and II, antigen-processing machinery, chemokines such as CXCL9/10/11, nitric-oxide and reactive-oxygen species, and hundreds of IFN-stimulated genes, thereby polarizing macrophages toward the microbicidal M1 phenotype, enhancing Th1 responses, orchestrating antiviral, antitumor and intracellular bacterial immunity, and exerting pro- or anti-inflammatory feedback depending on context; genetic or therapeutic blockade of IFNγ in mice and humans highlights its essential yet double-edged role in controlling infection, tumor surveillance, autoimmune diseases and immune-related adverse events.
免疫印迹
WB result of Invivo anti-Mouse IFNγ Recombinant mAb
Primary antibody: Invivo anti-Mouse IFNγ Recombinant mAb at 1/1000 dilution
Lane 1: IFN-γ Protein, mouse lysate 1 µg
Lane 2: IFN-γ Protein, human lysate 1 µg
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 15 kDa
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