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宿主来源
Human抗原名称
CD25分子别名
Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; IL2RA细胞定位
MembraneAccession
P01589抗体类型
Recombinant mAb抗体同种型
Human IgG1反应种属 ?
Hu纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CD25, also known as IL2RA, is the alpha chain of the interleukin-2 receptor, a heterotrimeric protein complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). It is a membranous glycoprotein primarily expressed on activated T lymphocytes and plays a crucial role in immune homeostasis by interacting with interleukin-2, a pleiotropic cytokine. CD25 is involved in the proliferation of T and B lymphocytes and is essential for the assembly of the high-affinity interleukin-2 receptor. In addition to its role in the immune system, CD25 is also expressed on certain non-immune cells and has been identified as a potential therapeutic target in cancers.
免疫印迹
WB result of Anti-Human IL2R Monoclonal Antibody (Basiliximab)
Primary antibody: Anti-Human IL2R Monoclonal Antibody (Basiliximab) at 1/1000 dilution
Lane 1: IL-2R alpha/CD25 His Tag Protein, Human lysate 1 µg
Lane 2: IL-2R alpha/CD25 His Tag Protein, mouse lysate 1 µg
Secondary antibody: Goat Anti-Human IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
ELISA
Immobilized IL-2R alpha/CD25 His Tag Protein, Human (UA010367) can bind Basiliximab. The EC50 for this effect is 3.312 ng/mL.
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