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Rat克隆号
S-3599抗体类型
Rat mAb纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
In vivo control
Isotype control antibodies, to estimate the nonspecific binding of target.
流式分析
Flow cytometric analysis of mouse thymocytes labeled with Rat IgG1 Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of mouse thymocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Rat IgG1 Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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