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Human克隆号
S-4796抗体类型
Recombinant mAb纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
In vivo control
Isotype control antibodies, to estimate the nonspecific binding of target.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Human IgG2, λ Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Human IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of C57BL/6 mouse splenocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Human IgG2, λ Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Human IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
多重免疫组化
Flow cytometric analysis of C57BL/6 mouse splenocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Human IgG2, λ Isotype Control antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Human IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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