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Rat抗原名称
CD80分子别名
T-lymphocyte activation antigen CD80; Activation B7-1 antigen (B7); B7; Cd80细胞定位
Cell membraneAccession
Q00609克隆号
1G10抗体类型
Rat mAb抗体同种型
Mouse IgG2a, κ同型对照
S0B0932反应种属 ?
Ms阳性样本
LPS treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo blocking
in vitro blocking
FCM
稀释度
应用 稀释度 推荐种属 FCM 1: 500 Ms
CD80，also known as B7-1，is a co - stimulatory molecule expressed on antigen - presenting cells（APCs）such as dendritic cells，macrophages and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a co - stimulatory signal necessary for T cell activation，thereby promoting T cell proliferation and differentiation. Additionally，CD80 can also bind to CTLA - 4，an inhibitory receptor on T cells，which acts as a negative regulator of T cell activation. This binding can inhibit the immune response and maintain immune tolerance. Moreover，CD80 - CD28 interaction is involved in the regulation of various immune - related processes，including the formation of immunological synapses and the regulation of cytokine production.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes, treated 3 days with 500 ng/ml LPS (right panel) or untreated (left panel), labeled with Mouse CD80 (B7-1) at 1/500 dilution (1 μg). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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