分享产品介绍 评论(0)
宿主来源
Rat抗原名称
CD24分子别名
Signal transducer CD24; Lymphocyte antigen 52 (Ly-52); M1/69-J11D heat stable antigen (HSA); Nectadrin; R13-Ag; X62 heat stable antigen; Cd24a; Ly-52; Cd24细胞定位
Cell membraneAccession
P24807克隆号
M1/69抗体类型
Rat mAb抗体同种型
Rat IgG2b, κ同型对照
Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo neutralization
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
CD24, also known as the cluster of differentiation 24, is a highly glycosylated glycosylphosphatidylinositol (GPI)-anchored surface protein that plays crucial roles in various physiological and pathological processes. Initially identified as a B-cell differentiation antigen, it is widely expressed in hematopoietic stem cells, B and T lymphocytes, epithelial cells, and neural cells. Structurally, CD24 has an intracellular domain, a transmembrane domain, and a heavily glycosylated extracellular domain, which is involved in cell adhesion, migration, differentiation, and apoptosis.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Mouse CD24 antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG2b, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
评论(0)