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宿主来源
Rat抗原名称
CD134分子别名
Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; Ox40; Txgp1; Tnfrsf4Accession
P47741克隆号
OX-86抗体类型
Rat mAb抗体同种型
Rat IgG1,k同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes treated 48h with 2.5 μg/ml Concanavalin A纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro cell specific stimulation/activation
in vivo neutralization
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
CD134, also known as TNFRSF4 or OX40, is a member of the tumor necrosis factor receptor superfamily. It is a co-stimulatory molecule primarily expressed on activated CD4+ and CD8+ T cells, as well as other immune cells such as natural killer (NK) cells and dendritic cells. Structurally, CD134 contains four TNFR-Cys repeats and interacts with its ligand, OX40L (TNFSF4), to activate downstream signaling pathways, including the NF-κB pathway through adaptor proteins like TRAF2 and TRAF5. This interaction promotes T cell proliferation, cytokine production, and enhances the survival of CD8+ T cells, playing a crucial role in immune responses. Additionally, CD134 has been implicated in various diseases, including cancer and autoimmune conditions, making it a target for immunotherapy and other therapeutic interventions.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes treated 48h with 2.5 μg/ml Concanavalin A (right panel) or untreated (left panel), labeled with CD134 at 1/500 dilution (1 μg). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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