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Mouse抗原名称
CD8a分子别名
T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; OX-8 membrane antigen; Cd8a细胞定位
Cell membraneAccession
P07725克隆号
OX-8抗体类型
Mouse mAb抗体同种型
Mouse IgG1, κ同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Rt阳性样本
SD rat splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD8+ T cell depletion
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Rt
CD8A, also known as T-cell surface glycoprotein CD8 alpha chain, is a protein encoded by the CD8A gene. It is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating efficient cell-cell interactions within the immune system. The CD8 protein acts as a coreceptor with the T-cell receptor on T lymphocytes to recognize antigens displayed by antigen-presenting cells in the context of class I MHC molecules. The functional coreceptor can be either a homodimer composed of two alpha chains or a heterodimer composed of one alpha and one beta chain. CD8A has significant homology to the variable domain of immunoglobulin light chains. It plays an essential role in the immune response, enabling cytotoxic T lymphocytes to recognize and eliminate infected cells and tumor cells.
流式分析
Flow cytometric analysis of SD rat splenocytes labelled Rat CD8a antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - APC antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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