分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD4分子别名
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; Cd4细胞定位
Cell membraneAccession
P05540克隆号
W3/25抗体类型
Mouse mAb抗体同种型
Mouse IgG1, κ同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Rt阳性样本
SD rat splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD4+ T cell depletion
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Rt
CD4 is a glycoprotein expressed on the surface of certain immune cells, most notably helper T cells. It is a member of the immunoglobulin superfamily and has four immunoglobulin-like domains (D1 to D4). CD4 acts as a co-receptor with the T-cell receptor (TCR) to recognize antigens displayed by MHC class II molecules on antigen-presenting cells. The D1 domain of CD4 binds to the β2 region of MHC class II, bringing the TCR complex and CD4 into close proximity. This allows the tyrosine kinase Lck, bound to the cytoplasmic tail of CD4, to phosphorylate tyrosine residues on the cytoplasmic domains of CD3, amplifying the signal generated by the TCR. CD4 is also the primary receptor for the human immunodeficiency virus (HIV), and its interaction with the HIV gp120 protein is a key step in viral entry.
流式分析
Flow cytometric analysis of SD rat splenocytes labelled Rat CD4 antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - APC antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
评论(0)