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Rat抗原名称
CD19分子别名
B-lymphocyte antigen CD19; Differentiation antigen CD19; Cd19细胞定位
Cell membraneAccession
P25918克隆号
1D3抗体类型
Rat mAb抗体同种型
Rat IgG2a, κ同型对照
Invivo rat IgG2a isotype control, anti-trinitrophenol反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD19 neutralization
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
CD19 is a 95 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is expressed on the surface of B lymphocytes and follicular dendritic cells. It plays a crucial role in B cell activation and humoral immune responses by forming a complex with CD21, CD81, and CD225. This complex reduces the threshold for B cell receptor (BCR) activation, enhancing downstream signaling pathways and facilitating B cell responses to antigens. CD19 is also a biomarker for B cell development and lymphoma diagnosis, and it is targeted in immunotherapies for diseases like leukemia.
流式分析
Flow cytometric analysis of Mouse CD19 expression on C57BL/6 mouse splenocytes. Cells were labeled with either Rat IgG2a, κ Isotype Control (black line histogram) or SDT Rat Anti-Mouse CD19 antibody (red line histogram) at 1/500 dilution (1 μg). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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