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宿主来源
Rat抗原名称
CD223分子别名
Lymphocyte activation gene 3 protein; LAG-3; Activation-induced cytidine deaminase-linked autoimmunity protein (Aida); Lag3细胞定位
Secreted, Cell membraneAccession
Q61790克隆号
C9B7W抗体类型
Rat mAb抗体同种型
Rat IgG1,k同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms阳性样本
Mouse CD3, CD28 antibody treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro blocking
Functional Assay
in vivo blocking
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
CD223, also known as LAG-3 (lymphocyte activation gene-3), is a type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on the surface of activated T cells, regulatory T cells (Tregs), natural killer (NK) cells, and some other immune cells. Structurally, it is similar to CD4 and has a higher binding affinity to MHC class II molecules than CD4. LAG-3 primarily functions as an immune checkpoint receptor, negatively regulating T-cell activation, proliferation, and cytokine production. In cancer and chronic infections, elevated LAG-3 expression is associated with T cell exhaustion, limiting anti-tumor and antiviral responses. Due to its significant role in immune regulation, LAG-3 has become a promising target for cancer immunotherapy and the treatment of autoimmune disorders.
流式分析
Flow cytometric analysis of mouse CD223 expression on mouse CD3 and CD28 antibody treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes untreated (left panel) or treated 72h with anti-mouse CD3, CD28 (right panel) were labeled with Mouse CD223 antibody at 1/500 dilution (1 μg). Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 488 antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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