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宿主来源
Armenian hamster抗原名称
CD272分子别名
B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; Btla细胞定位
Cell membraneAccession
Q7TSA3克隆号
6A6抗体类型
Recombinant mAb抗体同种型
IgG同型对照
Invivo Armenian Hamster IgG Isotype Control反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, Contains no stabilizers or preservatives
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
Functional assays
in vivo cell specific stimulation/activation
in vitro cell specific stimulation/activation
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
CD272 (BTLA) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with mouse CD272 antibody at 1/500 dilution (1 μg) / (right panel) compared with an Amenian hamster IgG Isotype Control / (left panel). Goat Anti- Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Alexa Fluor® 647 antibody separately. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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