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Mouse抗原名称
CD8α分子别名
T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; MAL; CD8A细胞定位
Cell membraneAccession
P01732克隆号
OKT-8抗体类型
Mouse mAb抗体同种型
Mouse IgG2a, κ同型对照
Invivo mouse IgG2a isotype control反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD8+ T cell depletion
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Hu
CD8α is a crucial immune glycoprotein that plays a vital role in the immune response by serving as a co-receptor for the T-cell receptor (TCR) on cytotoxic T cells. It forms either a homodimer (α/α) or a heterodimer with CD8β (α/β) to interact with MHC class I molecules on antigen-presenting cells (APCs), facilitating the recognition of intracellular pathogens such as viruses. CD8α also recruits the LCK kinase, which initiates downstream signaling pathways essential for T-cell activation and proliferation. This molecule is critical for the function of cytotoxic T lymphocytes (CTLs) and is involved in various immune-related diseases, including immunodeficiencies and autoimmune conditions.
流式分析
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labeled Human CD8a antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG2a Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ Antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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