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宿主来源
Rat抗原名称
CD314 (NKG2D)分子别名
NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; Nkg2d; Klrk1细胞定位
Cell membraneAccession
O54709克隆号
CX5抗体类型
Rat mAb抗体同种型
Rat IgG1,k同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD314 blockade
in vitro CD314 blockade
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
NKG2D is a type II transmembrane C-type lectin-like receptor expressed as a disulfide-linked homodimer on virtually all human and mouse NK cells, γδ T cells, and CD8+ αβ T cells; upon engagement by stress-inducible MHC class I–related ligands (MICA/B, ULBPs in humans; Rae-1, H60, Mult1 in mice) it couples to the DAP10 (and in mouse also DAP12) adaptor to deliver phosphotyrosine signals that powerfully lower activation thresholds, costimulate cytotoxicity, and drive proliferation and cytokine release, thereby forming a central surveillance axis that eliminates transformed, infected, or otherwise stressed cells but also contributes to immune-mediated pathologies when ligand expression becomes chronic.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Mouse CD314 antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG1, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with NK1.1 - Alexa Fluor® 647 antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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