Invivo anti-Human CD16 Recombinant mAb
Low affinity immunoglobulin gamma Fc region receptor III-A,IgG Fc receptor III-A,CD16-II,CD16a antigen,Fc-gamma RIII-alpha (Fc-gamma RIII,Fc-gamma RIIIa,FcRIII,FcRIIIa,FcgammaRIIIA),FcR-10,IgG Fc receptor III-2,CD16a,FCG3,FCGR3,IGFR3,FCGR3A
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宿主来源
Mouse抗原名称
CD16分子别名
Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; CD16a; FCG3; FCGR3; IGFR3; FCGR3A细胞定位
Secreted, Cell membraneAccession
P08637克隆号
3G8抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Hu阳性样本
Human peripheral blood lymphocytes纯化方式
Protein G浓度
5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro blocking
in vitro cell specific stimulation/activation
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Hu
CD16, also known as FcγRIII, is a type of glycoprotein belonging to the immunoglobulin superfamily. It is divided into two subtypes: CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a transmembrane protein mainly expressed on natural killer (NK) cells, monocytes, and some T cell subsets, while CD16b is a GPI-anchored protein primarily found on neutrophils. CD16a can mediate antibody-dependent cell cytotoxicity (ADCC) by binding to the Fc region of IgG antibodies, which is crucial for the immune system to eliminate virus-infected cells and cancer cells. Additionally, CD16a is one of the core markers for defining NK cells and can promote their proliferation and secretion of cytotoxic cytokines. In cancer immunotherapy, CD16a is an important target protein, and some monoclonal antibody drugs are designed to enhance its binding ability to boost immune responses.
流式分析
Flow cytometric analysis of human peripheral blood lymphocytes labelling Human CD16 antibody at 1/500 dilution (1 μg) / (right panel) was compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD56 - Phycoerythrin antibody separately. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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