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宿主来源
Mouse抗原名称
CD40分子别名
Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; TNFRSF5细胞定位
Secreted, Cell membraneAccession
P25942克隆号
G28.5抗体类型
Mouse mAb抗体同种型
Mouse IgG1, κ同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Hu阳性样本
Human Peripheral Blood cells纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
Functional assays
in vitro CD40 stimulation
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Hu
CD40, also known as TNFRSF5 or Bp50, is a 48 kDa type I membrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is widely expressed on various immune cells, including B cells, dendritic cells, and macrophages. The interaction between CD40 and its ligand, CD40L, is crucial for immune cell activation and regulation. Structurally, CD40 consists of a 62 amino acid cytoplasmic tail, a 22 amino acid transmembrane domain, and a 173 amino acid extracellular domain. This co-stimulatory protein plays a key role in B cell activation, proliferation, and differentiation, and is also involved in immune responses against infections and tumors. Due to its significant role in the immune system, CD40 has become an important therapeutic target, with various agonistic and antagonistic antibodies being developed to modulate its activity.
流式分析
Flow cytometric analysis of human peripheral blood cells labelling Human CD40 antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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