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宿主来源
Rat抗原名称
CD11b分子别名
Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Itgam细胞定位
Cell membraneAccession
P05555, P11215克隆号
M1/70抗体类型
Rat mAb抗体同种型
Rat IgG2b,k同型对照
Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD11b neutralization
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Hu, Ms
CD11b, also known as integrin alpha M (ITGAM), is a cell surface receptor belonging to the integrin family and primarily expressed on immune cells such as macrophages, monocytes, neutrophils, and NK cells. It forms a heterodimer with CD18 to create the β2 integrin complex, which is crucial for mediating cell adhesion, migration, and immune responses. CD11b plays a significant role in leukocyte recruitment and activation during inflammation and immune responses by interacting with ligands like ICAM-1 and iC3b. Additionally, it is involved in phagocytosis and immune regulation in various diseases, including cancer and autoimmune conditions.
流式分析
Flow cytometric analysis of C57BL/6 mouse bone marrow labelling CD11b antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG2b, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with Pacific blue™ Rat Anti-Mouse Ly-6G/Ly-6C antibody separately. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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