Invivo Anti-Mouse F4/80 Recombinant mAb
Adhesion G protein-coupled receptor E1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,EGF-like module-containing mucin-like hormone receptor-like 1,EMR1 hormone receptor,Emr1,Gpf480,Adgre1
分享产品介绍 评论(0)
宿主来源
Rat抗原名称
F4/80分子别名
Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1细胞定位
Cell membraneAccession
Q61549克隆号
CI:A3-1抗体类型
Rat mAb抗体同种型
Rat IgG2b,k同型对照
Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin反应种属 ?
Ms阳性样本
BMDM纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, Contains no stabilizers or preservatives
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
Functional assays
in vivo cell specific depletion
in vitro cell specific depletion
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
流式分析
Flow cytometric analysis of BMDM (Bone Marrow-Derived Macrophages) labelling F4/80 antibody at 1/500 dilution (1 μg) / (red) compared with a Rat IgG2b, κ (black) Isotype Control. Goat Anti-Rat IgG Alexa Fluor® 647 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
评论(0)