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Mouse抗原名称
CD28分子别名
T-cell-specific surface glycoprotein CD28; Cd28细胞定位
Cell membraneAccession
P31042克隆号
JJ319抗体类型
Mouse mAb抗体同种型
Mouse IgG1, κ同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Rt纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro T cell stimulation/activation
FCM
CD28 is a critical costimulatory receptor constitutively expressed on the surface of naïve T cells that, upon binding the ligands CD80 or CD86 on antigen-presenting cells, delivers an indispensable second signal that synergizes with TCR engagement to trigger full T-cell activation, proliferation, cytokine production (especially IL-2), metabolic reprogramming and survival by recruiting PI3K, GRB2/GADS and Lck to phosphorylate downstream effectors such as Akt, PKCθ and NF-κB, and its blockade or absence leads to anergy, whereas its engineered inclusion in CAR-T cells or therapeutic agonism is exploited to enhance anti-tumor immunity.
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