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Rat抗原名称
VEGF-A分子别名
Vascular endothelial growth factor A, long form; L-VEGF; Vascular permeability factor (VPF); Vegf; Vegfa细胞定位
SecretedAccession
Q00731克隆号
S-2820抗体类型
Rat mAb抗体同种型
Rat IgG2a,k同型对照
Invivo rat IgG2a isotype control, anti-trinitrophenol反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo VEGF-A neutralization
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
VEGF-A (Vascular Endothelial Growth Factor A) is a critical signaling protein that plays a central role in angiogenesis—the formation of new blood vessels from existing ones—by stimulating endothelial cell proliferation, migration, and vascular permeability, primarily through binding to its receptors VEGFR-1 and VEGFR-2 on endothelial cells, and is essential for normal physiological processes such as wound healing and embryonic development, while its dysregulation is implicated in pathological conditions including cancer, diabetic retinopathy, and age-related macular degeneration.
免疫印迹
WB result of Invivo anti-Mouse VEGF-A Recombinant mAb
Primary antibody: Invivo anti-Mouse VEGF-A Recombinant mAb at 1/1000 dilution
Lane 1: mouse VEGF-A protein 2 µg
Secondary antibody: Goat Anti- rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 25-35 kDa
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