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Mouse抗原名称
BTLA (CD272)分子别名
B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; Btla细胞定位
Cell membraneAccession
Q7TSA3克隆号
S-3831抗体类型
Mouse mAb抗体同种型
IgG1同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo BTLA blockade
in vivo BTLA stimulation
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:200 Ms
BTLA (CD272) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse BTLA (CD272) antibody at 1/200 dilution (1 μg) / (Right panel) compared with a Rat IgG1 Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Pacific Blue™ Antibody separately.
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