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宿主来源
Mouse抗原名称
CD47分子别名
Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein (IAP); Protein MER6; MER6细胞定位
Cell membraneAccession
Q08722克隆号
B6H12抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
Invivo mouse IgG1 isotype control反应种属 ?
Hu纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vitro CD47 neutralization
in vivo CD47 neutralization in human tumor xenograft models or humanized mice
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CD47, also known as integrin-associated protein, is a ubiquitously expressed cell surface receptor that belongs to the immunoglobulin superfamily. It is widely known for its role in preventing macrophage-mediated phagocytosis by interacting with signal regulatory protein alpha (SIRPα) on phagocytic cells. This “don’t eat me” signal is crucial for the survival of healthy cells, but cancer cells often overexpress CD47 to evade immune attack. In addition to its role in immune regulation, CD47 is involved in various cellular processes, including cell adhesion, migration, and the regulation of autophagy and cell death. For instance, it can regulate the migration of immune cells like neutrophils and dendritic cells, and its interaction with thrombospondin-1 (TSP-1) can induce apoptosis in certain cell types. Due to its significant role in cancer immunotherapy, CD47 has become an important target for developing new therapeutic strategies.
免疫印迹
WB result of Invivo anti-human CD47 Recombinant mAb
Primary antibody: Invivo anti-human CD47 Recombinant mAb at 1/1000 dilution
Lane 1: CD47 His Tag Protein, Mouse 10 ng
Lane 2: CD47 His Tag Protein, Human 10 ng
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 25-36 kDa
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