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Rat抗原名称
4-1BB (CD137)分子别名
Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB; Ila; Ly63; Tnfrsf9细胞定位
Cell membraneAccession
P20334克隆号
LOB12.3抗体类型
Rat mAb抗体同种型
Rat IgG1,k同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo 4-1BB stimulation
in vitro 4-1BB stimulation
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Ms
4-1BB (CD137, TNFRSF9) is a 39 kDa inducible costimulatory receptor expressed on activated T, NK, NKT and dendritic cells that, upon engagement by its trimeric ligand 4-1BBL or agonistic antibodies, recruits TRAF1/2 to trigger NF-κB, MAPK and PI3K–Akt signaling cascades, thereby enhancing proliferation, survival, cytokine production and cytotoxicity of CD8⁺ and CD4⁺ T cells and NK cells; its expression is tightly regulated (peaking 24–72 h post-activation and restrained by PD-1/CTLA-4 pathways) and its therapeutic agonism is exploited in immuno-oncology to overcome tumor-induced exhaustion, while antagonists are explored for autoimmune diseases.
免疫印迹
WB result of Invivo Anti-Mouse 4-1BB (CD137) Recombinant mAb
Primary antibody: Invivo Anti-Mouse 4-1BB (CD137) Recombinant mAb at 1/1000 dilution
Lane 1: CD137 Protein, Human 2 µg
Lane 2: CD137 Protein, Rat 2 µg
Lane 3: CD137 Protein, Mouse 2 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 25-35 kDa
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