分享产品介绍 评论(0)
宿主来源
Rat抗原名称
CD4分子别名
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; Cd4细胞定位
Cell membraneAccession
P06332克隆号
YTS 191抗体类型
Rat mAb抗体同种型
Rat IgG2b,k同型对照
Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo CD4+ T cell depletion
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
CD4 is a glycoprotein that functions as a co-receptor for the T-cell receptor (TCR) and is primarily expressed on the surface of helper T cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily, consisting of four immunoglobulin domains (D1 to D4) that interact with MHC class II molecules on antigen-presenting cells. This interaction, mediated by the D1 domain of CD4, ensures that T cells recognize antigens presented by MHC II. The cytoplasmic tail of CD4 contains motifs that recruit the tyrosine kinase Lck, which amplifies T cell activation signals. Additionally, CD4 is crucial in the immune response, as it helps activate CD4+ T helper cells, which in turn stimulate other immune cells to combat infections. However, CD4 is also the primary receptor for HIV, allowing the virus to enter host T cells and cause immunodeficiency.
免疫印迹
WB result of Invivo Anti-Mouse CD4 Recombinant mAb
Primary antibody: Invivo Anti-Mouse CD4 Recombinant mAb at 1/1000 dilution
Lane 1: CD4/LEU3 His Tag Protein, Human 10 ng
Lane 2: CD4 Protein, Mouse 10 ng
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate
评论(0)