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宿主来源
Armenian hamster抗原名称
NKG2D分子别名
NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CD314; Nkg2d; Klrk1细胞定位
Cell membraneAccession
O54709克隆号
S-3681抗体类型
Recombinant mAb抗体同种型
Armenian hamster IgG同型对照
Invivo Armenian Hamster IgG Isotype Control反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS, pH 7.4, Contains no stabilizers or preservatives
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo NKG2D blockade
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
NKG2D is a type II transmembrane C-type lectin-like receptor expressed as a disulfide-linked homodimer on virtually all human and mouse NK cells, γδ T cells, and CD8+ αβ T cells; upon engagement by stress-inducible MHC class I–related ligands (MICA/B, ULBPs in humans; Rae-1, H60, Mult1 in mice) it couples to the DAP10 (and in mouse also DAP12) adaptor to deliver phosphotyrosine signals that powerfully lower activation thresholds, costimulate cytotoxicity, and drive proliferation and cytokine release, thereby forming a central surveillance axis that eliminates transformed, infected, or otherwise stressed cells but also contributes to immune-mediated pathologies when ligand expression becomes chronic.
免疫印迹
WB result of Invivo Anti-Mouse NKG2D Recombinant mAb
Primary antibody: Invivo Anti-Mouse NKG2D Recombinant mAb at 1/1000 dilution
Lane 1: NKG2D/CD314 Fc Chimera Protein, Mouse 1 µg
Secondary antibody: Goat Anti- Armenian hamster IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48-56 kDa
This blot was developed with high sensitivity substrate
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