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Rat抗原名称
IL-10分子别名
Interleukin-10; Cytokine synthesis inhibitory factor (CSIF); Il-10; Il10细胞定位
SecretedAccession
P18893克隆号
JES5-2A5抗体类型
Recombinant mAb抗体同种型
Rat IgG1,k同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS, pH 7.4, Contains no stabilizers or preservatives
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo lL-10 neutralization
in vitro lL-10 neutralization
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Ms
Interleukin-10 (IL-10) is an 18 kDa anti-inflammatory cytokine composed of 160 amino acids folded into two intrachain disulfide-linked α-helical dimers that signals through the heterotetrameric IL-10R1/IL-10R2 receptor complex to activate JAK1/TYK2-mediated STAT3 phosphorylation, thereby down-regulating major histocompatibility class II, co-stimulatory molecules and pro-inflammatory cytokines such as TNF-α, IL-1, IL-6, IL-8, IL-12 and IFN-γ, while simultaneously up-regulating IL-1RA and soluble TNF receptors, ultimately exerting potent immunosuppressive and tissue-protective functions in both innate and adaptive immunity.
免疫印迹
WB result of Invivo Anti-Mouse IL-10 Recombinant mAb
Primary antibody: Invivo Anti-Mouse IL-10 Recombinant mAb at 1/1000 dilution
Lane 1: IL-10 Protein, Human 1 µg
Lane 2: IL-10 Protein, Mouse 1 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 17-27 kDa
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