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Mouse抗原名称
PD-L1分子别名
Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; B7 homolog 1 (B7-H1); B7H1; PDCD1L1; PDCD1LG1; PDL1; CD274细胞定位
Cell membraneAccession
Q9NZQ7克隆号
29E.2A3抗体类型
Recombinant mAb抗体同种型
Mouse IgG2b,k反应种属 ?
Hu纯化方式
Protein A浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
Functional Assay
in vitro PD-L1 blockade
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
PD-L1 is a ~33 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily that functions as the key ligand for the inhibitory receptor PD-1 on T cells; upon IFN-γ–driven up-regulation on dendritic cells, macrophages, normal epithelia and many tumors, its engagement of PD-1 delivers a “stop” signal that blocks T-cell receptor signaling, suppressing proliferation, cytokine production and cytotoxic activity, thereby maintaining peripheral tolerance and preventing autoimmunity, but is exploited by cancers to evade immune destruction, making the PD-1/PD-L1 axis a central target for modern checkpoint-inhibitor immunotherapies.
免疫印迹
WB result of Invivo Anti-Human PD-L1 (B7-H1) Recombinant mAb
Primary antibody: Invivo Anti-Human PD-L1 (B7-H1) Recombinant mAb at 1/1000 dilution
Lane 1: PD-L1/B7-H1 Protein, Mouse 1 µg
Lane 2: PD-L1 Protein, Human 1 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 35 kDa
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