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Mouse抗原名称
CD25分子别名
Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA细胞定位
MembraneAccession
P01589克隆号
7G7B6抗体类型
Recombinant mAb抗体同种型
Mouse IgG2a, κ同型对照
Invivo mouse IgG2a isotype control反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
5 mg/ml纯度
>95%(Determined by SDS-PAGE)内毒素含量
<1EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
in vivo regulatory T cell depletion in humanized mice
FCM
ELISA
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu FCM 1:250 Hu
CD25, also known as IL2RA, is the alpha chain of the interleukin-2 receptor, a heterotrimeric protein complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). It is a membranous glycoprotein primarily expressed on activated T lymphocytes and plays a crucial role in immune homeostasis by interacting with interleukin-2, a pleiotropic cytokine. CD25 is involved in the proliferation of T and B lymphocytes and is essential for the assembly of the high-affinity interleukin-2 receptor. In addition to its role in the immune system, CD25 is also expressed on certain non-immune cells and has been identified as a potential therapeutic target in cancers.
流式分析
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labelling Human CD25 (IL-2Rα) antibody at 1/250 dilution (2 μg) / (Right panel) compared with a Mouse IgG2a Isotype Control / (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.Then cells were stained with CD4 - Brilliant Violet 421™ Antibody separately.
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