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Mouse抗原名称
MHC Class I (H-2Kb)分子别名
H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1Accession
P01901克隆号
Y-3抗体类型
Mouse mAb抗体同种型
Mouse IgG2b,k反应种属 ?
Ms纯化方式
Protein A浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
in vivo MHC I blockade
Functional Assay
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:500 Ms
MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse MHC Class I (H-2Kb) antibody at 1/500 (1 μg) dilution (Right panel) compared with a Mouse IgG2b, κ Isotype Control (Left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Brilliant Violet 421™ antibody separately.
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